Recombinant ErbB2/Her2 anticorps (Extracellular Domain)
Aperçu rapide pour Recombinant ErbB2/Her2 anticorps (Extracellular Domain) (ABIN7477489)
Antigène
Voir toutes ErbB2/Her2 AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
-
-
Épitope
- Extracellular Domain
-
Immunogène
- Recombinant protein encoding the extracellular domain of human c-erbB2
-
Isotype
- IgG
-
-
-
-
Indications d'application
- IHC 1:50-1:200
-
Commentaires
-
Positive Control: Colon: Breast cancers with HER2 amplification should show strong positivity. Placenta: A faint staining should be seen at the surface membrane of the syncytiotrophoblast.
Negative Control: Breast: Normal epithelial cells must not show HER2 immunostaining.
-
Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Stock
- 4 °C,-20 °C,-80 °C
-
Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))
-
Autre désignation
- HER2 / CD340
-
Sujet
- P185, CD340, Verb b2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog,HER2 / CD340 antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P04626
-
Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development
Antigène
-